TABLE 2.
Phosphoryl group donor specificitya
| Phosphoryl donorb | Relative activity (%) |
|---|---|
| Poly(P)x | 100 |
| AMP | 0 |
| ADP | 0 |
| ATP | 75 |
| GTP | 111 |
| CTP | 76 |
| TTP | 13 |
| UTP | 94 |
| ITP | 89 |
| Meta(P)x | 87 |
| Orthophosphate | 0 |
| Pyrophosphate | 0 |
| Poly(P)3 | 11 |
| Poly(P)5 | 22 |
| Poly(P)18 | 87 |
| Poly(P)27 | 101 |
| Poly(P)32 | 90 |
| Poly(P)46 | 67 |
| Poly(P)62 | 52 |
a
The specific activity obtained in an assay mixture composed of 100 mM bis-Tris-HCl (pH 6.8), 5 mM NAD, 5 mM MnCl2, and 2 mg/ml poly(P)x is represented as 100%.
b
The final concentration used was 5 mM, except for poly(P)x and meta(P)x, for which the final concentration was 2 mg/ml.