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Effect of cations on NAD kinase activitya
| Cationb | Poly(P)x-dependent relative activity (%) | ATP-dependent relative activity (%) |
|---|---|---|
| None | 0 | 0 |
| MnCl2 | 100 | 62 |
| MgCl2 | 103 | 103 |
| NiCl2 | 80 | 36 |
| CaCl2 | 57 | 65 |
| ZnCl2 | 56 | 78 |
| CuCl2 | 100 | 158 |
The specific activity obtained in an assay mixture composed of 100 mM bis-Tris-HCl (pH 6.8), 5 mM NAD, 5 mM MnCl2, and 2 mg/ml poly(P)x is represented as 100%.
The concentration of cations used was 5 mM.
