Figure 2.

Plasmodesmal localization of calreticulin requires signal peptide. A, Hydropathy profile of AtCRT1. Hydrophobic domains are indicated by a positive index and hydrophilic domains by a negative index (Kyte and Doolittle, 1983). Dotted rectangle delineates the signal sequence. B, AtCRT1-GFP transiently expressed in N. benthamiana. C, AtCRT1-del50N-GFP lacking its signal peptide transiently expressed in N. benthamiana. D, AtCRT2-YFP stably expressed in transgenic Arabidopsis from its full-length gene with native regulatory elements. E, TMV MP-GFP stably expressed in transgenic Arabidopsis. YFP, CFP, and GFP signals are indicated in green, and plastid autofluorescence is indicated in red. Images in B and D are single confocal sections; images in C and E are projections of several confocal sections. Bars = 10 μm.