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. 2005 Aug;138(4):1866–1876. doi: 10.1104/pp.105.064386

Figure 4.

Figure 4.

Interaction between calreticulin and TMV MP and effect of calreticulin overexpression on TMV MP subcellular localization. A, Interaction in yeast two-hybrid system. Lane 1, AtCRT1 + TMV MP; lane 2, AtCRT1 + lamin C; lane 3, AtCRT1 lacking signal peptide + TMV MP. B, RT-PCR analysis of ZmCRT1 overexpression in transgenic N. benthamiana. Lane 1, Plants transgenic for ZmCRT1; lane 2, wild-type plants. Top and bottom bands correspond to the ZmCRT1- and actin-specific products. Fragment size in base pairs is indicated on the right. C, Western-blot analysis of ZmCRT1 overexpression in transgenic plants. Lane 1, Plants transgenic for ZmCRT1; lane 2, wild-type plants. Molecular mass expressed in thousands of Daltons is indicated on the right. D, Interaction in renatured-blot overlay assay. Lane 1, Plants transgenic for ZmCRT1 + TMV MP; lane 2, wild-type plants + TMV MP; lane 3, plants transgenic for ZmCRT1 + VirD2. Arrowhead indicates the position of calreticulin. Molecular mass expressed in thousands of Daltons is indicated on the right. E, GFP-TMV MP transiently expressed in wild-type N. benthamiana. F, GFP-TMV MP transiently expressed in transgenic N. benthamiana plants overexpressing ZmCRT1. GFP signal is in green; plastid autofluorescence is in red. Images in E and F are projections of several confocal sections; plastid autofluorescence in E was filtered out. Bars = 10 μm.