Figure 6.

AtPex16p exists in Arabidopsis root cell peroxisomes. A and B, Representative confocal optical sections of Arabidopsis root cells dual labeled with rabbit PA anti-AtPex16p IgGs (1:100, overnight) and mouse monoclonal anti-catalase IgGs (1:2, overnight). Hand-sectioned roots from 4-week-old plants were fixed in formaldehyde, digested partially in a mixture of pectinase and cellulase, and cell membranes were permeabilized in Triton X-100. A, Punctate immunofluorescence (Cy2) pattern of endogenous AtPex16p (arrows). B, Superimposable punctate peroxisomal catalase immunofluorescence (Cy5) pattern in the same cells (arrows). Bar = 5 μm.