Figure 10.

Enhanced expression of GA biosynthetic genes in the BR-deficient mutant brd1-2 and suppression by BL treatment. Quantitative RT-PCR was performed using primers specific for each GA biosynthetic gene, as described in “Materials and Methods.” Plants were treated with (+) 10⁻⁶ m BL or control solution (−), as described in “Materials and Methods.” The expression levels of OsGA20ox2/SD1 (A), OsGA20ox1 (B), OsGA3ox2/D18 (C), and OsKAO (D) were enhanced in brd1-2 and suppressed by BL treatment.