Figure 9.

Degradation of SLR1 triggered by GA₃ treatment of dgl1. Protein gel-blot analysis of SLR1 in young seedlings of the wild type, dgl1-3, and the GA-insensitive mutant gid2-1. The plants were treated with (+) 10⁻⁴ m GA₃ or control solution (−), as described in “Materials and Methods.” The arrow indicates the protein bands corresponding to endogenous SLR1. Each lane contains 10 μg of total protein. The loading control of Coomassie Brilliant Blue staining is shown.