Figure 4.

Alexander staining reveals that a SEC8 allele with an absolute transmission defect is associated with a pollen germination defect. A to E, Staining phenotypes of wild-type pollen grains in vivo, illustrating (from left to right, ungerminated to empty grain) a likely progression of cytoplasm (stained purple) out of the grain and into the growing pollen tube. F and G, Representative fields of stained pollen grains from a wild-type homozygote (F) and a sibling sec8-m3 heterozygote (G). White arrows, Ungerminated grains; stigma cells stain green. H, Quantitation of in vivo germination rates from three different pairs of lines, assayed blindly 4 h after pollination. Hatched bars, Mutants; white bars, wild-type siblings.