Figure 6.

RNAi-mediated silencing of ARP7 expression. A, The ARP7Ri construct contains an inverted repeat of 300 bp of ARP7-exon 1 (see Fig. 2A) as the target sequence and a 1-kb partial GUS gene as the linker sequence. The cauliflower mosaic virus-35S promoter (35S P) and nopaline synthase terminator (NOST) control expression from this construct. B, Western-blot analysis of selective knockdown of ARP7 expression. Top, middle, and bottom images were probed with mAbARP7a, mAbARP4, and mAbGPa, respectively. WT, Wild-type; 7Ri, an ARP7Ri line exhibiting distinct morphological phenotypes. Equal amounts of total protein from inflorescence samples were loaded per each lane. Note the drastic reduction in the level of ARP7, but not ARP4 or actin proteins, in the ARP7Ri plant.