Figure 8.

Molecular complementation of the cyp51A2-3 mutant by ectopic expression of CYP51A2. The homozygous cyp51A2-3 mutant containing the 35S::CYP51A2 transgene (right) has no differences in plant stature and fertility compared with wild-type (left) and transgenic plants overexpressing CYP51A2 (middle). F₂ plants from the crosses between the heterozygous cyp51A2-3 mutants and the homozygous transgenic plants overexpressing CYP51A2 were selected from Murashige and Skoog medium containing kanamycin (for selection of cyp51A-3 mutant) and gentamycin (for selection of the transgenic plants containing 35S::CYP51A2). The plants resistant to both antibiotics were subjected to the genotyping by PCR.