Table IV.

Oligonucleotides used for gene cloning and for the semiquantitative PCR analysis

Underlined sequences correspond to enzyme restriction sites. F, Forward oligonucleotide; R, reverse oligonucleotide.

Name	Oligonuclotide Sequence (5′ to 3′)
Adaptor primer 1	CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGGAGGT
Adaptor primer 2	Ph-ACCTCCCC-NH
MDAR-GEN-F	TTAGGGGGCAAATATACTAAGTTC
MDAR-BamHI F	TTATTGGATCCGTGCATTCGTTC
MDAR-EcoRI-F	TTACGGAATTCGTGCATTCGTTC
MDAR-SQ-F	AACGTGTGGTTGTTGGAGG
MDAR-SQ-F	AAAGGGACAGTTGCTGTTGG
MDAR-GSP2	TGCATAACCAGCTGAAACTCCTC
MDAR-GSP1	CCCAGGATGAACTCCTTGTTTCAC
R-MDAR-Bam	TACAGGATCCATCCTTCCCAAGA
MDAR-GEN-R	ACACAGCGTAAAGCATATCCCACAC
ACT-SQ-F	AGCAAGATCCAAACGAAGGA
ACT-SQ-R	AATGGTGAAGGCTGGATTTG