Figure 4.

Import of soybean rps10 mutants into mitochondria and outer membrane ruptured mitochondria. The import of four mutants was tested into intact mitochondria and outer membrane ruptured mitochondria. Mutants O and P were imported into intact mitochondria as they were protease protected (lanes 1–3). This import was not sensitive to addition of valinomycin (lanes 4 and 5). Mutants O and P were not imported across or into the inner membrane in outer membrane ruptured mitochondria (lanes 6–9). Mutants Q and R were imported into intact mitochondria in a valinomycin sensitive manner (lanes 1–5) and were imported into outer membrane ruptured mitochondria in a membrane potential dependent manner (lanes 6–9).