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. 2005 Aug;138(4):2220–2232. doi: 10.1104/pp.105.062083

Table IV.

Comparison between maize Dea and W22 incubated with Glc with or without starvation pretreatment

Maize root tips were incubated with [U-14C]Glc and [1-13C]Glc (see “Materials and Methods”) after starvation pretreatment (maize root tips were incubated 4 h without Glc and then 25 h plus labeled Glc) or not. For Dea, with starvation pretreatment, the ratio C-1 to C-6 and the fluxes were calculated from data published by Dieuaide-Noubhani et al. (1995). All fluxes, Vres, Vgpase, and Vi were determined as for W22 (see “Results”). The values of relative fluxes were calculated for a rate of Glc consumption (Vg) equal to 1. The values of absolute fluxes were then calculated for Vg equal to 350 and 394 nmol min1 g1 FW for Dea and W22, respectively. Results are given as mean ± sd (n = 5 for W22 without starvation pretreatment, and n = 2 for W22 with starvation pretreatment). For Dea without starvation pretreatment, results are from one experiment.

Without Starvation Pretreatment
With Starvation Pretreatment
Dea W22 Dea W22
Enrichment ratio C-1 to C-6
    Sucrose glucosyl 4.6 4.3 ± 0.4 5.7 5.9 ± 0.4
    Sucrose fructosyl 3.8 3.7 ± 0.3 5.4 5.0 ± 0.2
    Glucose 6.5 5.1 ± 0.5 8.6 8.1 ± 0.4
Relative fluxes
    Vres 2.9 6.86 ± 1.33 2.1 3.0 ± 0.2
Absolute fluxes (μmol min−1 g−1 FW)
    Vres 1.0 2.70 ± 0.52
    Vi 0.2 0.15 ± 0.03
    Vgpase 0.8 2.55 ± 0.52