Comparison between maize Dea and W22 incubated with Glc with or without starvation pretreatment
Maize root tips were incubated with [U-14C]Glc and [1-13C]Glc (see “Materials and Methods”) after starvation pretreatment (maize root tips were incubated 4 h without Glc and then 25 h plus labeled Glc) or not. For Dea, with starvation pretreatment, the ratio C-1 to C-6 and the fluxes were calculated from data published by Dieuaide-Noubhani et al. (1995). All fluxes, Vres, Vgpase, and Vi were determined as for W22 (see “Results”). The values of relative fluxes were calculated for a rate of Glc consumption (Vg) equal to 1. The values of absolute fluxes were then calculated for Vg equal to 350 and 394 nmol min−1 g−1 FW for Dea and W22, respectively. Results are given as mean ± sd (n = 5 for W22 without starvation pretreatment, and n = 2 for W22 with starvation pretreatment). For Dea without starvation pretreatment, results are from one experiment.