Figure 10.

Effects of in vitro GB on temperature-dependent association of Rubisco activase with the thylakoid membranes. A, Soluble fractions. B, Thylakoid fractions. Soluble fractions and thylakoid fractions were isolated from the broken chloroplasts, which were incubated without or with exogenous GB (equivalent to 1.5 μmol mg⁻¹ chlorophyll) at different temperatures for 10 min. The broken chloroplasts were obtained after an osmotic shock from intact chloroplasts of wild-type plants. C, Quantitation of results in A and B. The values are mean ± se of three independent experiments.