Figure 4.

Analysis of native HSP90C complexes by BN-PAGE. Chlamydomonas total soluble proteins from cells grown in continuous light (CL) and from cells that, in the light, were exposed for 1 h to a heat shock (HS) at 40°C, were separated on a 6% to 15% polyacrylamide gradient blue native gel (BN-PAGE), and either transferred to nitrocellulose (central gel) or separated in the second dimension on 10% SDS-polyacrylamide gels (SDS-PAGE), followed by transfer to nitrocellulose (left and right gels; note that the top of the second dimension gel is shown facing the BN-PAGE gels at the center of the figure). Membranes were immunodecorated with an antibody against HSP90C.