Figure 2.

ACC treatment impairs stomatal closure induced by ABA treatment only when plants can transmit the ethylene signal. A to C, Stomatal apertures of wild-type, eto1-1, ein3-1, and etr1-1 plants (A), 1-MCP-treated wild-type and eto1-1 plants (B), and AVG-treated wild-type and eto1-1 plants (C), in the preopened condition (white bars), after incubation in darkness for 2 h (gray bars), after incubation with 10 μm ABA for 2 h in the light (black bars), and after incubation with 10 μm ABA and 10 μm ACC in the light (striped bars). The data are representative of three independent experiments, showing the means of 100 stomata. Bars represent means ± ses.