Figure 5.

Delay in stomatal closure of eto1-1 and wild-type plants exposed to ethylene compared to wild-type plants under drought stress. A, Expression patterns of an ABA-induced gene, RAB18, were compared between before and after detachment. mRNA was extracted from leaves of wild-type plants before detachment (white bars) and 30 min after detachment (black bars). Gene expression levels were measured by real-time quantitative PCR, and the relative amounts of transcripts then calculated and normalized with actin7 mRNA. Bars represent the means ± ses (n = 4–8). B, Time-course changes in stomatal apertures after detachment of wild type (white circles), wild type exposed to ethylene gas (black squares), and eto1-1 plants (black circles). C, Overall phenotype of wild type, eto1-1, and wild-type plant exposed to gaseous ethylene 30 min after detachment (from left to right). D to K, Stomata of wild-type plants before detachment of leaves (D), wild-type plants 5 min after leaf detachment (E), eto1-1 plants before detachment (F), eto1-1 plants 5 min (G) or 30 min after detachment (H), and wild type exposed to ethylene gas before detachment (I) and 5 min (J) or 30 min (K) after detachment, as observed by optical microscopy (×400; bars = 20 μm).