Fig. 2.

FOXA1, a target of ERα, is recruited to a subset of ERα targets. (A) Pie chart representing major biological functions and processes associated with ERα targets (153) enriched in E₂-treated MCF-7 cells. (B) Induction of FOXA1 expression by E₂ as monitored by Western blot. (C) FOXA1 recruitment to a subset of ERα-bound promoters containing FOXA1 binding sites as assayed by conventional ChIP. The STS and HK1 promoters serve as a ERα-bound control promoters without a FOXA1-binding site. The results presented are from a single experiment representative of three independent experiments.