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. 2005 Jun 7;388(Pt 3):745–754. doi: 10.1042/BJ20041807

Figure 3. Expression of DrZIP1 protein in cultured cells and its subcellular localization in CHSE-214 cells.

Figure 3

(A) Detection of DrZIP1(H)6 protein expressed in Caco-2, MDCK and CHSE-214 cells by SDS-Urea/PAGE and Western blotting. Lanes 1, 3 and 6: DrZIP1(H)6-expressed cells; lane 4: stable MDCK-DrZIP1(H)6 cells; and lanes 2, 5 and 7: negative control cells transfected with pIRES2EGFP. (B) Subcellular localization of DrZIP1 proteins expressed in CHSE-214 cells. The plasmid construct, pCI-neo-DrZIP1EGFP, was transfected into CHSE-214 cells, and the subcellular localization of the expressed DrZIP1EGFP protein in CHSE-214 cells was examined with an inverted fluorescent microscope. a and d, bright light; b and e, blue light; c and f, overlapping of pictures from both bright and blue light.