(A) Luciferase activities in CHSE-214 cells that were co-transfected with zinc-reporter plasmid, pMT-793, and one among pIRES2EGFP-DrZIP1, pIRES2EGFP-FrZIP2, pIRES2EGFP-FrECaC and pIRES2EGFP, and subsequently treated with 30 μM ZnSO4 for 24 h. (B) Luciferase activities in CHSE-214 cells, that were co-transfected with pMT-793 and either pCI-neo-DrZIP1 or pCI-neo, and subsequently treated with 30 μM ZnSO4 for 24 h. Values are means±S.E.M. (n=3). Significant differences between experimental groups (P<0.05) were determined using Mann–Whitney U test.