Table 1. Kinetic parameters of A. dirus GSTs.
The data for adGSTD1-1, adGSTD2-2, adGSTD3-3, adGSTD4-4 and adGSTD6-6 have been reported previously [6,8,11].
| CDNB | GSH | |||||
|---|---|---|---|---|---|---|
| Isoenzyme | Vmax (μmol/min per mg) | kcat (s−1) | Km (mM) | kcat/Km (mM−1·s−1) | Km (mM) | kcat/Km (mM−1·s−1) |
| adGSTD5-5 | 167±48 | 69 | 14.9±4.9 | 5 | 0.21±0.01 | 400 |
| adGSTD1-1 | 12.9±0.6 | 5.03 | 0.10±0.03 | 48.4 | 0.86±0.2 | 5.86 |
| adGSTD2-2 | 63.9±3.50 | 25.9 | 0.21±0.03 | 121 | 1.30±0.15 | 20 |
| adGSTD3-3 | 67.5±1.97 | 26.9 | 0.10±0.01 | 269 | 0.40±0.05 | 67 |
| adGSTD4-4 | 40.3±1.89 | 16.9 | 0.52±0.67 | 32 | 0.83±0.08 | 20 |
| adGSTD6-6 | 2.2±0.3 | 0.9 | 5.3±0.8 | 0.2 | 1.8±0.4 | 0.5 |