Table 1. Purification of spinach leaf ketoamine kinase.

Step	Volume (ml)	Protein (g)	Total activity (m-unit)*	Specific activity (unit/g)	Purification	Yield (%)
Leaf extract	200	6.1	2819	0.5		100
25% PEG pellet	50	2.9	1590	0.5	1	56
DEAE-Sepharose	17	0.12	873	7	15	31
Q-Sepharose	10	0.01	342	34	74	12
Sephacryl S-200	7	0.001	183	174	379	6

* One unit of enzyme is the amount that catalyses the phosphorylation of one μmol of ribuloselysine/min in the presence of 100 μM ribuloselysine.