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Plasmids purified from E. coli or P. aeruginosa wild-type strains were digested with MboI or Sau3AI restriction enzymes, which are sensitive and insensitive to adenine methylation respectively, and then separated on 0.8% agarose gel. DNA was visualized by ethidium bromide staining. The negative of the photograph is shown. C, control plasmid from E. coli or P. aeruginosa not digested.
