Figure 3. Hydrolysis of β-1,3-xylan by rXYL4.

The pattern for the hydrolysis of β-1,3-xylan by rXYL4 was analysed by TLC. The reaction mixture containing 0.5% (w/v) β-1,3-xylan and 150 ng of enzyme in 30 μl of 50 mM sodium phosphate buffer, pH 7.5, was incubated at 37 °C for the periods indicated. After incubation, the reaction mixtures were centrifuged and the supernatants were analysed by TLC as described in the Experimental section.