Table 1.

Genotype and Percentage Chimerism for the Mice Used in This Study

^aPercentage wild-type based on PCs was estimated from calculating the density of PCs in five sections at least 100 μm apart. Standard deviation is in parentheses.

^bPercentage wild-type based on granule cells was estimated from counting of anti-NeuN-stained granule cells in three 65 × 65 μm regions from two sections at least 100 μm apart.

^cSevere ataxia is defined as a balance beam score of two or less. Homozygous mutant ages are the average of three male and three female mice.

^dThis small percentage of non-GFP PCs is likely an artifact from the thin sectioning; if a small fraction of a PC is in the section, it may not be scored GFP-positive. Examination of the same PC in the adjacent section shows that all PCs are in fact GFP-positive.

^eOnly lobule X PCs were used in estimating the wild-type contribution in mutant chimeric mice sacrificed at 70 d, prior to degeneration of this lobule.

^fND indicates not determined, as this mouse was sacrificed prior to developing severe ataxia.

^gNM indicates not meaningful, as lobule X PCs are only a useful measure of wild-type contribution when mice are sacrificed by 70 d.

CD1, CD-1 strain (Charles River Laboratories); C57, C57BL/6J strain (Jackson Laboratory).