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. 2005 Jul 28;102(32):11266–11271. doi: 10.1073/pnas.0408771102

Fig. 4.

Fig. 4.

Macronutrient storage in ogt-1ok(430). N2 and ogt-1(ok430) strains were assayed for their storage of glycogen, trehalose, and glucose as described in Materials and Methods. (A Left) Levels of each of the stored carbohydrates is shown. A 2.5- to 3-fold elevation in sugar storage was observed; this representative experiment was repeated nine times with nearly identical results. (A Right)(Lower) Carminic acid was detected by fluorescence microscopy and was predominantly found in the intestine of the animal. The carminic acid-positive structures are a distinct subset of granules in the intestinal cells in both N2 and ogt-1(ok430). (Upper) A corresponding Nomarski image is shown. (B Left) Storage of neutral lipids in N2 and ogt-1(ok430) was quantified in three replicate experiments as described in Materials and Methods. The reduction in neutral lipid storage was ≈70%. (B Right) (Lower) Enlargement of the anterior intestinal cells of the ogt-1(ok430) strain that was allowed to take up Nile red to label lipid droplets. (Upper) A corresponding Nomarski image is shown.