Fig. 2.

DNA sequence alignments (within boxes) showing the splice sites in the fused region containing mating-type genes MAT1-1 and MAT1-2 in self-fertile species of Stemphylium. The fused regions are aligned with their unfused homologs from other species. ORF1 is a flanking gene of unknown function (7). The top part of the diagram is an ORF1 proximal splice junction; bottom part is an ORF1 distal junction. The sequences are arranged by phylogenetic groups as in Fig. 3. Dots indicate nucleotide identity to P2. In the upper sequence box, the column of spaces indicates the junction between the forward flank and the inverted MAT1-1 region, where sequence homology between fused and unfused regions is lost. The shaded nucleotide positions indicate the ATGG that would, through inversion, produce a CCAT motif. In the lower sequence box, the shaded CCAT is the postulated crossover site with MAT1-1 to the left and MAT1-2 to the right.