Fig. 4.

Alignment of the RCL of chicken clade B serpins and human orthologues. Human SERPINB8 and -B9 are included for comparison. Shown at the top is the canonical numbering proximal or distal to the specificity-determining scissile bond (P₁–P₁′, boxed). The consensus hinge region of inhibitory serpins is E at P₁₇, E/K/R at P₁₆, G at P₁₅, T/S at P₁₄, small aliphatic residues (A/G/S) at P₁₂-P₉ (8), and T/SatP₈ (25). Residues are colored according to type: polar uncharged (green), acidic (red), basic (blue), and nonpolar (yellow). Information on the inhibitory activity of human ov-serpins is reviewed in ref 12. Of note, chicken ovalbumin and human SERPINB5/maspin are not protease inhibitors.