Fig. 1.

HSV transport between the cornea and ganglia and construction of an HSV US9^- mutant (US9^- HSV). (A) Cartoon depicting HSV infection spread from the cornea epithelium to the trigeminal (TG) ganglia, followed by anterograde spread to the corneal stroma. Sensory neurons possess both retrograde and anterograde modes of transport; however, for simplicity, the neuron projecting to the epithelium is shown only with retrograde transport. (B) The HSV-1 genome, including US8, US9, and US10 genes, with the US9 gene replaced with GFP sequences. (C) Western blot showing the loss of US9 in US9^- HSV-infected cells. gD is a second HSV membrane protein.