Fig. 2.

US9^- HSV spreads normally in epithelial cells and in mouse cornea. (A) Cultured ARPE-19 epithelial cells were infected with WT, US9^-, US9^-R, or gE^- HSV for 48 h, and then the cells were fixed and stained with rabbit anti-HSV antibodies and secondary fluorescent antibodies. (B) Mice were infected with HSV by corneal scarification; corneas were removed after 2 days and stained by using rabbit anti-HSV antibodies and secondary fluorescent antibodies. (White bar, 100 μm.)