Fig. 3.
US9- HSV spreads in neuronal axons in a retrograde but not in an anterograde direction. (A) Groups of five mice were infected with HSV (5 × 105 pfu) by corneal scarification. Corneas and TG ganglia were removed at day 4, homogenized, and pooled within each group, and infectious HSV was assayed by plaque titration. (B) Spread of HSV from the retina to optic centers of the brain was assessed by injecting each virus (2 × 105 pfu) into the vitreous body of the eye. After 5 days, brain sections were stained for viral antigens as in Fig. 2B. SC, superior colliculus; LGN, lateral geniculate nucleus; SCN, suprachiasmatic nucleus. (C) Mice were infected with HSV (3 × 105 pfu) by scarification of the snout. TG ganglia and corneas were removed after 5 days, and infectious HSV was measured in plaque assays.