Fig. 2.

Htt inclusions in shortstop and YAC128 mice at 18 months and AF at 12 months. EM48 inclusions labeled with FITC (green) are present in NeuN-stained neurons labeled with Texas red in the striatum (A) and cortex (C) of YAC128 mice, but not in the hippocampus (E). Inclusions are present in most cells in the striatum (B), cortex (D), and hippocampus (F) in the shortstop (SS) mouse. At 12 months of age, YAC128 cortex (G) and hippocampus (K) demonstrate less labeling of AF compared with shortstop cortex (H) and hippocampus (L). Few AF are present in the striatum of the YAC128 (I) or shortstop (J) mice. Inclusions are indicated with white arrowheads; AF are indicated with black arrowheads. (Scale bar in D, which applies to A-D:10 μm; scale bar in F, which apples to E and F: 20 μm; scale bar in H, which applies to G-L: 20 μm.)