Fig. 1.

Identification of MCPs in adult mouse bone marrow. (A) Lin^-c-Kit⁺Sca-1^-Ly6c^-FcεRIα^- cells were subdivided into β7⁺CD27^-, β7^-CD27^-, and β7^-CD27⁺ populations (red, blue, and green, respectively) and analyzed for their surface expression of T1/ST2, CD9, and β1 integrin. (B) Double-sorted β7^-CD27^-, β7⁺T1/ST2⁺ (MCPs), and β7⁺T1/ST2^- cells were cultured with SCF, EPO, TPO, Flt-3L, GM-CSF, and IL-1, -3, -6, -7, -9, and -11 and harvested at days 5 and 11 for flow cytometry analysis for mast cells to be detected by expression of c-Kit and FcεRIα.(C) Cytospin of double-sorted β7⁺T1/ST2⁺ (MCPs) and β7⁺T1/ST2^- cells at isolation (Upper) and after 11 days in culture (Lower) and May Grunwald-Giemsa staining of mast cells (arrows) and macrophages (arrowheads). (Scale bars, 10 μm.) (D) Day 10 results of colony-forming assay in methylcellulose containing SCF, EPO, TPO, Flt-3L, GM-CSF, and IL-1, -3, -6, -7, -9, and -11. Data shown are representative of those obtained in three (A, C, and D) or four (B) experiments. MC, mast cells; Mix, mixed; GM, granulocyte/macrophage; E/MK, erythrocyte/megakaryocyte.