Fig. 2.

CRIK expression is down-modulated with differentiation. (A) Expression of different Rho effectors was assessed in keratinocytes under growing conditions and at various times (hours) of calcium-induced differentiation by immunoblot analysis with antibodies against the indicated proteins. Immunoblotting for RasGAP was used as equal loading control. (B) mRNA levels of CRIK were evaluated in keratinocytes under growing versus differentiating conditions by Northern blot analysis with a CRIK specific probe as previously described (8). The same membrane was stripped and rehybridized with a GAPDH probe as equal loading control. (C) Frozen skin sections from newborn mice were analyzed by immunofluorescence with anti-CRIK rabbit antibodies (Upper) or affinity-purified Rabbit serum as nonimmune control (Lower).