Figure 5.

(A) Metabolites in rectal environment that changed relative to pre-infection (day −2) levels on days 3 and 9 post-infection (when bats were still shedding detectable vRNA). Colour indicates log₂-fold change relative to day −2 values; size of dot indicates –log(p). Analysis was performed separately for each diet group owing to differences in metabolite levels pre-infection. Infected bats were also compared with uninfected bats on each day post-infection to adjust for metabolic changes over the span of the experiment. (B) Metabolites from the rectal environment correlated with viral AUC for each bat pre-infection (day −2) and days 3 and 9 post-infection. Diet was not considered in this calculation and each infected bat was treated individually. Colour indicates positive correlation with AUC (i.e. higher levels of metabolite correlated with higher total virus shed) versus negative correlation with AUC. Size of dots indicates –log(p). Vertical red dotted line denotes pre- versus post-infection days. DPI, days-post-infection