Fig. 2.

RTN3 deficiency aggravated pulmonary fibrosis mice model induced by bleomycin and age. A RTN3 protein levels in the lung tissues of Wild type mice and RTN3-null mice were detected by Western blot. B HE staining and Masson staining showed the pathological changes of lung tissues for Wild type mice and RTN3-null mice treated with PBS or bleomycin on day 21. ELISA detection showed the SP-A levels (C) and SP-B (D) levels in the BALF isolated from Wild type mice and RTN3-null mice treated with PBS or bleomycin on day 21. E Western blot analysis exhibited the protein levels of Fn1, p-SMAD3, SMAD3, α-SMA and RTN3 levels in the lung tissues from Wild type mice and RTN3-null mice treated with PBS or bleomycin on day 21. F HE staining and Masson staining showed the pathological changes of lung tissues for Wild type mice and RTN3-null mice at 16 months old. ELISA detection showed the SP-A levels (G) and SP-B (H) levels in the BALF isolated from Wild type mice and RTN3-null mice at 16 months old