Fig. 3.

RTN3 reduction can promote the synthesis of collagen. A Significantly differentially expressed genes between Wild type and RTN3 KO mice lung tissues revealed by RNA-seq data. B Real-time PCR detected the RNA levels of collagen related genes in lung tissues of Wild type and RTN3 KO mice. Western blot analysis showed the COL1A1 levels in lung tissues of Wild type and RTN3 KO mice (C), primary cultured lung fibroblasts from Wild type and RTN3 KO mice (D) and MRC5 cell lines transfected with or without shRNA (E). ELISA detection exhibited the hydroxyproline levels in primary cultured lung fibroblasts from Wild type and RTN3 KO mice (F) and MRC5 cell lines transfected with or without shRNA (G)