Fig. 4.

RTN3 deficiency reduced the antagonism of ER-anchored CRTH2 on collagen synthesis in lung fibroblasts. A, B Co-IP showed the interaction between RTN3 with CRTH2 in primary cultured lung fibroblasts. Primary cultured lung fibroblasts were transfected with MYC-RTN3 plasmids. C Immunofluorescence staining showed the colocalization between RTN3 and CRTH2 in primary lung fibroblasts and MRC5 cell lines. D Immunofluorescence staining showed subcellular localization of RTN3 and CRTH2 in Wild type and RTN3-null primary lung fibroblasts. E Western blot analysis showed the expression of CRTH2 in ER in in Wild type and RTN3-null primary lung fibroblasts. Real-time PCR showed the stability of COL1A1 mRNA and COL1A2 mRNA in Wild type and RTN3-null primary lung fibroblasts (F), as well as in RTN3-null primary lung fibroblasts transfected with pcDNA3.1( +)-CRTH2 (G). H Western blot analysis showed the levels of COL1A1 and LARP6 in RTN3-null primary lung fibroblasts transfected with or without si-LARP6