Fig. 5.

Whole-exome sequencing identified a loss-of-function mutations in the RTN3 gene in the Chinese ILD population. A High resolution computed tomography of the patients who carried RTN3 mutations. B Sanger sequencing of RTN3 confirmed the c.548A > G/p.E183G mutation. C Immunofluorescence staining showed subcellular localization of Wild type and mutated RTN3. D Immunofluorescence staining showed subcellular localization of CRTH2 in RTN3-null lung fibroblasts transfected with Wild type and mutated RTN3 plasmids. E ELISA detection exhibited the hydroxyproline levels in RTN3-null lung fibroblasts transfected with Wild type and mutated RTN3 plasmids