Fig. 6.

RTN3 deficiency promote the profibrotic macrophage differentiation via reducing CRTH2 levels. A IHC showed the CD206 levels in lung tissue of mice treated by bleomycin or PBS on day 21. B Real-time PCR detected the mRNA levels of CD206, Arf1 and Fizz1 in alveolar macrophages isolated from Wild type and RTN3-null mice. C Flow cytometry detection showed the expression of CD206 in alveolar macrophages isolated from Wild type and RTN3-null mice. D Western blot analysis showed the levels of CRTH2, DNM2, Beclin1 and LC3A/B in alveolar macrophages isolated from Wild type and RTN3-null mice. E Immunofluorescence staining showed the colocalization between CRTH2 (red) and autophagy marker DNM2 (greed) in alveolar macrophages isolated from Wild type and RTN3-null mice