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[Preprint]. 2025 Jan 28:2025.01.28.635252. [Version 1] doi: 10.1101/2025.01.28.635252

Fig. 5: Co-cultured astrocytes respond to reduction in neuronal cholesterol receptor expression.

Fig. 5:

a, PCA of the normalized read counts in astrocytes co-cultured with LRP1-KD, LDLR-KD and NT-KD neurons across PC1 and PC2 explaining 8.54% and 7.02% of variation, respectively. b, Volcano plots of differential expression analysis comparing astrocytes grown with LRP1-KD neurons or LDLR-KD neurons to the astrocytes co-cultured with NT-KD neurons. Pink fill highlights the significant (adjusted p-value < 0.05) genes. c-d, Scatter plots of logCPM values from the DE analysis of the astrocytes co-cultured with LRP1-KD, LDLR-KD and NT-KD neurons. Diagonal (y = x) is indicated by a black line. Correlation coefficients (R) and p-values were computed using Pearson correlation. e, Boxplots of normalized expression (logCPM) of Nrxn1 and Scd2 in astrocytes co-cultured with LRP1-KD, LDLR-KD and NT-KD neurons. The p-values are calculated using DREAM linear mixed models. The boxplots’ limits show the upper- and lower quartile ranges, the center lines show the median values. The whiskers show the minimum and maximum values.