Extended Data Fig. 4: Validation of transcriptional changes in co-cultured neurons and astrocytes.
Gene expression trends of selected genes at various time points after co-culture of human NPCs and mouse glia, along with the corresponding ANOVA test p-value. The human neurons showed significant upregulation of synaptic genes SVB2 and SYT1. Thebon mouse glia showed significant upregulation of synaptic adhesion genes Nrxn1 and Nlgn1 as well as Srebf2, a gene implicated in cholesterol biosynthesis.
