Extended Data Figure 1. Evaluation of sSNV burden in control and IHD cardiomyocytes with the consideration of sequencing quality and amplification evenness metrics.

a-d, Covariate-adjusted sSNV burden in each single cardiomyocyte. Cardiomyocytes originating from the same individuals were shown with the same age. sSNV burden robustly exhibited a clear age-related accumulation trend and significant increases in IHD cardiomyocytes compared to controls, after adjusting for CoV (a), MAPD (b), coverage (c), and depth (d) as covariate (linear mixed-effects model). e, Consistent sSNV burden estimates between the original and refined SCAN2 methods. f, Some cells that failed to have their sSNV burden estimated in the original SCAN2 method were recovered using the refined method, showing similar burdens as other cells originating from the same individuals.