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. 1978 Feb 15;170(2):407–413. doi: 10.1042/bj1700407

Intestinal neuraminidase activity of suckling rats and other mammals. Relationship to the sialic acid content of milk.

J J Dickson, M Messer
PMCID: PMC1183908  PMID: 25066

Abstract

1. The neuraminidase activity of homogenates of the mucosa of the middle and distal thirds of the small intestine of rats increased about 5-fold between birth and 4 to 8 days of age, and then gradually declined to the much lower adult activity by 24 days. No comparable changes occurred in the proximal third. 2. In 8-day-old rats, the neuraminidase activity of the middle and distal thirds of the small intestine was about 10 times greater than that of the proximal third, 20 times greater than that of the colon and at least 100 times greater than that of the liver, brain, gastric mucosa or pancreas. 3. In all other species investigated (mice, rabbits, cats and guinea pigs), the neuraminidase activity of the middle and distal thirds of the small intestine was greater in suckling animals than in adults. 4. The sialic acid content of rat milk increased about 2-fold between birth and 8 days post partum and then declined. 5. There was a highly significant positive correlation between the intestinal neuraminidase activity of suckling animals of various species and ages and the sialic acid content of milk obtained from the corresponding species and stage of lactation. 6. It is suggested that the intestinal neuraminidase of suckling mammals functions primarily to remove sialic acid from various components of milk, thus providing sialic acid for the synthesis of sialoglycoproteins and gangliosides by the young.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADA G. L. Purification and properties of avian neuraminidase. Biochim Biophys Acta. 1963 Jun 11;73:276–284. doi: 10.1016/0006-3002(63)90312-3. [DOI] [PubMed] [Google Scholar]
  2. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alpers D. H. Separation and isolation of rat and human intestinal beta-galactosidases. J Biol Chem. 1969 Mar 10;244(5):1238–1246. [PubMed] [Google Scholar]
  4. Asp N. G., Dahlqvist A. Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration. Biochem J. 1968 Feb;106(4):841–845. doi: 10.1042/bj1060841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CARUBELLI R., TRUCCO R. E., CAPUTTO R. Neuraminidase activity in mammalian organs. Biochim Biophys Acta. 1962 Jun 18;60:196–197. doi: 10.1016/0006-3002(62)90392-x. [DOI] [PubMed] [Google Scholar]
  6. CLARK S. L., Jr The ingestion of proteins and colloidal materials by columnar absorptive cells of the small intestine in suckling rats and mice. J Biophys Biochem Cytol. 1959 Jan 25;5(1):41–50. doi: 10.1083/jcb.5.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clarke R. M., Hardy R. N. The use of [125-I] polyvinyl pyrrolidone K. 60 in the quantitative assessment of the uptake of macromolecular substances by the intestine of the young rat. J Physiol. 1969 Sep;204(1):113–125. doi: 10.1113/jphysiol.1969.sp008902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cornell R., Padykula H. A. A cytological study of intestinal absorption in the suckling rat. Am J Anat. 1969 Jul;125(3):291–315. doi: 10.1002/aja.1001250304. [DOI] [PubMed] [Google Scholar]
  9. Danovith S. H., Laster L. The development of arylsulphatase in the small intestine of the rat. Biochem J. 1969 Sep;114(2):343–350. doi: 10.1042/bj1140343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ghosh N. K., Kotowitz L., Fishman W. H. Neuraminidase in human intestinal mucosa. Biochim Biophys Acta. 1968 Aug 27;167(1):201–204. doi: 10.1016/0005-2744(68)90295-7. [DOI] [PubMed] [Google Scholar]
  11. HERINGOVA A., JIRSOVA V., KOLDOVSKY O. POSTNATAL DEVELOPMENT OF BETA-GLUCURONIDASE IN THE JEJUNUM AND ILEUM OF RATS. Can J Biochem. 1965 Feb;43:173–178. [PubMed] [Google Scholar]
  12. HSU L., TAPPEL A. L. LYSOSOMAL ENZYMES OF RAT INTESTINAL MUCOSA. J Cell Biol. 1964 Nov;23:233–240. doi: 10.1083/jcb.23.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Harrison R., Higginbotham J. D., Newman R. Sialoglycopeptides from bovine milk fat globule membrane. Biochim Biophys Acta. 1975 May 21;389(3):449–463. doi: 10.1016/0005-2736(75)90156-x. [DOI] [PubMed] [Google Scholar]
  14. Jumawan J., Koldovsky O., Palmieri M. Postnatal changes of -galactosidase activity in the small intestine of the rat. Biol Neonate. 1972;20(5):380–384. doi: 10.1159/000240480. [DOI] [PubMed] [Google Scholar]
  15. KOLDOVSKY O., CHYTIL F. POSTNATAL DEVELOPMENT OF BETA-GALACTOSIDASE ACTIVITY IN THE SMALL INTESTINE OF THE RAT. EFFECT OF ADRENALECTOMY AND DIET. Biochem J. 1965 Jan;94:266–270. doi: 10.1042/bj0940266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KORNFELD S., KORNFELD R., NEUFELD E. F., O'BRIEN P. J. THE FEEDBACK CONTROL OF SUGAR NUCLEOTIDE BIOSYNTHESIS IN LIVER. Proc Natl Acad Sci U S A. 1964 Aug;52:371–379. doi: 10.1073/pnas.52.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Keenan T. W. Membranes of mammary gland. IX. Concentration of glycosphingolipid galactosyl and sialytransferases in Golgi apparatus from bovine mammary gland. J Dairy Sci. 1974 Feb;57(2):187–192. doi: 10.3168/jds.s0022-0302(74)84858-7. [DOI] [PubMed] [Google Scholar]
  18. Kikuchi K., Kikuchi H., Tsuiki S. Activities of sialic acid-synthesizing enzymes in rat liver and rat and mouse tumors. Biochim Biophys Acta. 1971 Nov 12;252(2):357–368. doi: 10.1016/0304-4165(71)90017-1. [DOI] [PubMed] [Google Scholar]
  19. Kishore C. S., Tulsiani D. R., Bhavanandan V. P., Carubelli R. Membrane-bound neuraminidases of rat liver. Neuraminidase activity in Golgi apparatus. J Biol Chem. 1975 Apr 10;250(7):2655–2659. [PubMed] [Google Scholar]
  20. Koldovský O., Herbst J. N-acetyl-beta-glucosaminidase in the small intestine and its changes during postnatal development of the rat. Biol Neonate. 1971;17(1):1–9. doi: 10.1159/000240296. [DOI] [PubMed] [Google Scholar]
  21. Koldovský O., Palmieri M., Jumawan J. Comparison of activities of acid -galactosidase, -glucuronidase, N-acetyl- -glucosaminidase and -galactosidase in jejunum and ileum of adult and suckling rats. Comp Biochem Physiol B. 1972 Sep 15;43(1):1–8. doi: 10.1016/0305-0491(72)90193-9. [DOI] [PubMed] [Google Scholar]
  22. Kolodny E. H., Kanfer J., Quirk J. M., Brady R. O. Properties of a particle-bound enzyme from rat intestine that cleaves sialic acid from Tay-Sachs ganglioside. J Biol Chem. 1971 Mar 10;246(5):1426–1431. [PubMed] [Google Scholar]
  23. Kuhn N. J. The lactose and neuraminlactose content of rat milk and mammary tissue. Biochem J. 1972 Nov;130(1):177–180. doi: 10.1042/bj1300177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  25. LUCKEY T. D., MENDE T. J., PLEASANTS J. The physical and chemical characterization of rat's milk. J Nutr. 1954 Nov 10;54(3):345–359. doi: 10.1093/jn/54.3.345. [DOI] [PubMed] [Google Scholar]
  26. Mahadevan S., Nduguba J. C., Tappel A. L. Sialidase of rat liver and kidney. J Biol Chem. 1967 Oct 10;242(19):4409–4413. [PubMed] [Google Scholar]
  27. Messer M., Kerry K. R. Milk carbohydrates of the echidna and the platypus. Science. 1973 Apr 13;180(4082):201–203. doi: 10.1126/science.180.4082.201. [DOI] [PubMed] [Google Scholar]
  28. Roukema P. A., Van Den Eijnden D. H., Heijlman J., Van Der Berg G. Sialoglycoproteins, gangliosides and related enzymes in developing rat brain. FEBS Lett. 1970 Aug 31;9(5):267–270. doi: 10.1016/0014-5793(70)80373-8. [DOI] [PubMed] [Google Scholar]
  29. Tulsiani D. R., Carubelli R. Studies on the soluble and lysosomal neuraminidases of rat mammary glands. Biochim Biophys Acta. 1971 Jan 13;227(1):139–153. doi: 10.1016/0005-2744(71)90175-6. [DOI] [PubMed] [Google Scholar]
  30. Williams R. M., Beck F. A histochemical study of gut maturation. J Anat. 1969 Nov;105(Pt 3):487–501. [PMC free article] [PubMed] [Google Scholar]
  31. Woodward D. R., Messer M. Chemical composition of rat casein. Comp Biochem Physiol B. 1976;55(1):141–143. doi: 10.1016/0305-0491(76)90187-5. [DOI] [PubMed] [Google Scholar]

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