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Analysis of the H-P-T axis of the WT and E457A mutant mice. (A–C) Total serum T4, T3, and TSH levels (n = 13–34). (D and E) Pituitary Tshb and liver Gsta mRNA levels of TR-βWT/WT, TR-βE457A/WT, and TR-βE457A/E457A mice according to quantitative RT-PCR analysis. Data were normalized for each mRNA level relative to values for the WT animals (n = 6–10). Data are shown as mean ± SEM.
