Figure 3.

Lipid accumulation in livers of L-Insig1^–/–Insig2^–/– mice. Adult female mice were treated with 4 intraperitoneal injections of pIpC (300 μg/injection), and the livers were removed either 12 days (A and B) or 7 days (C and D) after the final injection. (A and B) Photographs of livers from chow-fed control and L-Insig1^–/–Insig2^–/– mice. (C and D) Oil red O–stained histologic sections of the livers from chow-fed control (left) and L-Insig1^–/–Insig2^–/– mice. Mice were perfused through the heart with HBSS and then with 10% (v/v) formalin in PBS; frozen sections of livers were stained with oil red O. Magnification, ×20.