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. Author manuscript; available in PMC: 2005 Aug 10.
Published in final edited form as: J Biol Chem. 2004 Jul 27;279(39):41179–41188. doi: 10.1074/jbc.M400292200

Fig. 7.

Fig. 7

Ang-3 is colocalized with perlecan but not with syndecan-1 or glypican-1. LLCAng-3 (A–F) and TA3Ang-3 (G–I) cells were fixed, and the relative localization of Ang-3v5 and syndecan-1 were detected with FITC-conjugated anti-v5 mAb (A, green fluorescence) or TRITC-conjugated anti-syndecan-1 antibody (B, red fluorescence), respectively. Panels A and B were merged to show that there is little colocalization between Ang-3v5 and syndecan-1 (C). The relative localizations of glypican-1 (D, green fluorescence) and Ang-3v5 (E, red fluorescence) were revealed by anti-glypican-1 antibody with FITC-conjugated secondary antibody and anti-v5 mAb with TRITC-conjugated secondary antibody, respectively. Panels D and E were merged to show that there is little colocalization between Ang-3v5 and glypican-1 (F). The relative localizations of Ang-3v5 (G, green fluorescence) and perlecan (H, red fluorescence) in the fixed TA3Ang-3 cells were revealed by anti-v5 mAb with FITC-conjugated secondary antibody and anti-perlecan antibody with TRITC-conjugated secondary antibody, respectively. Panels G and H were merged to show that Ang-3v5 and perlecan are colocalized on surface of these cells (I). Bar, 35 μm.