Figure 6.

Compound 45 facilitated cell apoptosis in A549 cells. (A) Annexin V-FITC/PI double staining assay was performed to evaluate the apoptotic effect of 45 on A549 cells after treatment with the indicated concentrations of compound 45 for 48 h. (B) The quantitative histograms of the apoptosis percentage in A549 cells. (C–F) Cells were treated with the indicated concentrations of compound 45 for 48 h and then analyzed for apoptosis-related protein expression by Western blotting. Each bar represents the mean ± SD (n = 3); *p < 0.05, **p < 0.01, or ***p < 0.001 was considered statistically significant compared with corresponding control values.