Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1978 Jan 1;169(1):87–94. doi: 10.1042/bj1690087

Vitamin A receptors. Retinoic acid binding in ocular tissues.

B Wiggert, D R Bergsma, R Helmsen, G J Chader
PMCID: PMC1184197  PMID: 415739

Abstract

Analysis of the sucrose-density-gradient patterns of the 110 000g supernatant fractions of adult and foetal retina and pigment epithelium showed them to contain a limited number of highly specific binding sites ('receptors') for [3H]retinoic acid that sediment at approx. 2S. Binding in pigment epithelium is higher than in any tissue yet reported. A 5S binding component is also observed and is probably due to serum contamination. Fractionation studies indicate that [3H]retinoic acid binding in the retina is lower in the photoreceptor units than in the retinal inner layers. This is in contrast with previous results that show greater [3H]retinol binding in photoreceptors. Studies with dystrophic human and rat retinas, which lack the photoreceptor layers, confirm that [3H]retinoic acid binding is greater in the non-photoreceptor layers of the retina. No specific [3H]retinoic acid binding is found in corneal epithelium, although endothelium and the conjunctiva demonstrate specific 2S binding. Such differences in retinol and retinoic acid binding may indicate different roles for the two compounds in ocular tissues.

Full text

PDF
89

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergsma D. R., Wiggert B. N., Funahashi M., Kuwabara T., Chader C. J. Vitamin A receptors in normal and dystrophic human retina. Nature. 1977 Jan 6;265(5589):66–67. doi: 10.1038/265066a0. [DOI] [PubMed] [Google Scholar]
  2. FELL H. B. The effect of excess vitamin A on cultures of embryonic chicken skin explanted at different stages of differentiation. Proc R Soc Lond B Biol Sci. 1956 Mar 26;146(923):242–256. doi: 10.1098/rspb.1957.0008. [DOI] [PubMed] [Google Scholar]
  3. Futterman S., Saari J. C., Swanson D. E. Retinol and retinoic acid-binding proteins in bovine retina: aspects of binding specificity. Exp Eye Res. 1976 May;22(5):419–424. doi: 10.1016/0014-4835(76)90180-9. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Ong D. E., Chytil F. Changes in levels of cellular retinol- and retinoic-acid-binding proteins of liver and lung during perinatal development of rat. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3976–3978. doi: 10.1073/pnas.73.11.3976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ong D. E., Chytil F. Retinoic acid-binding protein in rat tissue. Partial purification and comparison to rat tissue retinol-binding protein. J Biol Chem. 1975 Aug 10;250(15):6113–6117. [PubMed] [Google Scholar]
  7. Saari J. C., Futterman S. Retinol-binding protein in bovine retina: isolation and partial characterization. Exp Eye Res. 1976 May;22(5):425–433. doi: 10.1016/0014-4835(76)90181-0. [DOI] [PubMed] [Google Scholar]
  8. Saari J. C., Futterman S. Separable binding proteins for retinoic acid and retinol in bovine retina. Biochim Biophys Acta. 1976 Oct 22;444(3):789–793. doi: 10.1016/0304-4165(76)90325-1. [DOI] [PubMed] [Google Scholar]
  9. Sporn M. B., Dunlop N. M., Newton D. L., Smith J. M. Prevention of chemical carcinogenesis by vitamin A and its synthetic analogs (retinoids). Fed Proc. 1976 May 1;35(6):1332–1338. [PubMed] [Google Scholar]
  10. THOMPSON J. N., HOWELL J. M., PITT G. A. VITAMIN A AND REPRODUCTION IN RATS. Proc R Soc Lond B Biol Sci. 1964 Feb 18;159:510–535. doi: 10.1098/rspb.1964.0017. [DOI] [PubMed] [Google Scholar]
  11. Wiggert B. O., Bergsma D. R., Chader G. J. Retinol receptors of the retina and pigment epithelium: further characterization and species variation. Exp Eye Res. 1976 May;22(5):411–418. doi: 10.1016/0014-4835(76)90179-2. [DOI] [PubMed] [Google Scholar]
  12. Wiggert B. O., Chader G. J. A receptor for retinol in the developing retina and pigment epithelium. Exp Eye Res. 1975 Aug;21(2):143–151. doi: 10.1016/0014-4835(75)90078-0. [DOI] [PubMed] [Google Scholar]
  13. Wiggert B., Bergsma D. R., Helmsen R. J., Alligood J., Lewis M., Chader G. J. Retinol receptors in corneal epithelium, stroma and endothelium. Biochim Biophys Acta. 1977 Mar 28;491(1):104–113. doi: 10.1016/0005-2795(77)90045-9. [DOI] [PubMed] [Google Scholar]
  14. Wiggert B., Bergsma D. R., Lewis M., Chader G. J. Vitamin A receptors: retinol binding in neural retina and pigment epithelium. J Neurochem. 1977 Dec;29(6):947–954. doi: 10.1111/j.1471-4159.1977.tb06498.x. [DOI] [PubMed] [Google Scholar]
  15. Wolfe L. S., Kin N. M., Baker R. R., Carpenter S., Andermann F. Identification of retinoyl complexes as the autofluorescent component of the neuronal storage material in Batten disease. Science. 1977 Mar 25;195(4284):1360–1362. doi: 10.1126/science.841336. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES